Technology
At Insingulo, we believe that the best way to study the protein targets that are essential in many diseases, is to study the targets in their natural form. Our guiding principle is to deliver technology that is flexible enough to accommodate even the most difficult targets in their natural state. With other methods, it is necessary to manipulate and change these targets to adapt to the assay which reduces the viability of the results.
The context
Not only can our technology accommodate targets in their native state, but it can also detect single binding events. This allows you to measure the kinetics of screening compounds completely free from labels that can impede natural interactions.
Our technology gives you the flexibility to investigate your targets in a natural state, giving you data
that you can rely on. Our technology is validated in screening for membrane proteins, intrinsically
disordered proteins, PPIs, fragments, and much more.
Membrane proteins have an essential role in regulating physiological processes making them a prime target for therapeutic drugs in many different diseases. However, the most common methods for studying proteins can often not be used with membrane proteins which are much more challenging to work with due to their transmembrane domains. InSingulo presents a new and simple way to investigate membrane proteins as drug targets, unlocking new opportunities in drug discovery.
The challenge of membrane proteins
The InSingulo solution
In essence, InSingulo combines the advantages of assays that conserve the biological context of a target, with the advantages of label-free biosensing.
- Tim Kaminski, Chief Scientific Officer
The Benefits of the InSingulo Technology
Conserves nature
High specificity
High sensitivity
Single binding events, as detected by InSingulos technology, are the smallest increment of a binding
reaction, making our technology highly sensitive. Another advantage of InSingulo’s technology is that the signal levels are independent of the test compounds molecular weight.
Low reagent constraints
In contrast to other inhibition in solution assays, InSingulo’s technology allows using the use of
target definition compounds (TDC) with a broad affinity range from sub-nanomolar to micromolar.
Another difference with InSingulo’s technology is is that as a result of its single-molecule resolution,
the observed kinetics of the test compounds are not influenced by the binding kinetics of the TDC.
Free from this limitation, the same TDC can be used throughout the entire development process from screening to end of lead optimization.
The technology is applicable to soluble and membrane proteins. For membrane proteins, the technology is well suited to handle a huge variety of samples giving the scientists the freedom they need.